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KMID : 0545119980080010074
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 1 p.74 ~ p.80
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Characteristics of the Conditioned Medium Produced by CA-12 Lymph Node Stromal Cells
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Lee, Sang Han
Lee, Dong Sun/Seu, Young Bae/Kim, Jong Guk/Takashi Tsuruo/Hong, Soon Duck
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Abstract
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CS-21 lymphoma cells that preferentially metastasize to lymph nodes after s.c. inoculation into BALB/c mice were grown in vitro in the presence of CA12 stromal cells isolated from lymph nodes. In order to obtain fundamental data on the identification and characterization of the soluble factors produced by CA-12 stromal cells, the conditioned medium of CA-12 stromal cells that inhibited apoptosis of CS-21 cells was examined. Various analytical treatments revealed that the soluble factors in CA-12 conditioned medium are very sensitive to heat treatment and trypsinization. Moreover CA-12 conditioned medium has an affinity with heparin but not with Con-A. In addition to these, the activity of CA-12 conditioned medium was blocked by H-7, a PKC inhibitor, but the conditioned medium could not induce the differentiation of thymocytes. We concluded that CA-12 conditioned medium contains stromal cell-derived apoptosis-inhibitory molecules that play an important role in proliferation of CS-21 cells by suppressing cell apoptosis.
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KEYWORD
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